重组人IL-13

产品货号：

GS4874

中文名称：

重组人IL-13

英文名称：

Recombinant Human Interleukin-13

产品规格：

10μg

发货周期：

1～3天

产品价格：

询价

General Description:

Human Interleukin-13 (IL-13) is expressed by the IL13 gene located on the chromosome 5 and secreted by many cell types,especially T helper type 2 (Th2) cells.The high solution from of IL-13 reported to be a monomer with two internal disulfide bonds that contribute to a bundled four α-helix configuration.Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites.IL-13 exerts anti-inflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1beta,TNF-alpha,IL-6 and IL-8.IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching resulting in increased production of IgE.Human,mouse and rat IL-3 share low homology,but have cross species activity.

Storage:

This lyophilized preparation is stable at 2～8℃,but should be kept at -20℃ for long term storage,preferably desiccated.Upon reconstitution,the preparation is stable for up to one week at 2～8℃.For maximal stability,apportion the reconstituted preparation into working aliquots and store at -20℃ to -70℃.Avoid repeated freeze/thaw cycles.

Usage:

This material is offered by bjbalb Biotech for research,laboratory or further evaluation purposes.
NOT FOR HUMAN USE.

Molecular Weight:

Approximately 12.5kDa,a single non-glycosylated polypeptide chain containing 112 amino acids.

Purity:

> 97% by SDS-PAGE and HPLC analyses.

Source:

Escherichia coli.

Biological Activity:

Fully biologically active when compared to standard.The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 1ng/mL,corresponding to a specific activity of> 1.0×106 IU/mg.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Lyophilized from a 0.2μm filtered concentrated solution in PBS,pH7.4 with 5% trehalose.

Endotoxin:

Less than 1 EU/μg of rHuIL-13 as determined by LAL method.

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1～1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃.Further dilutions should be made in appropriate buffered solutions.

AA Sequence:

GPVPPSTALR ELIEELVNIT QNQKAPLCNG SMVWSINLTA GMYCAALESL INVSGCSAIE KTQRMLSGFC PHKVSAGQFS SLHVRDTKIE VAQFVKDLLL HLKKLFREGR FN

产品目录